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DNA扩增、二代测序、分子诊断
℡ 4000-520-616
℡ 4000-520-616
Kapa biosystems/KAPA Library Amplification Kits/KK2702/250 x 50 µL reactions
产品编号:KK2702
市  场 价:¥13980.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$699.00
品      牌: Kapa biosystems
公      司:Kapa Biosystems.
公司分类:
Kapa biosystems/KAPA Library Amplification Kits/KK2702/250 x 50 µL reactions
商品介绍

Description:

Real-timePCRlibraryamplification,withfluorescentstandards

KAPALibraryAmplificationKits

ThegoldstandardforNGSlibraryamplification.

KAPALibraryAmplificationKitscontainKAPAHiFiDNAPolymerase,anovelenzymeengineeredforultra-highfidelityandrobustnessusingKapa’sdirectedevolutiontechnologyplatform.

KAPAHiFihasbecometheenzymeofchoiceforNGSlibraryamplificationduetoitsABIlitytoamplifycomplexDNApopulationswithhighfidelity,highefficiency,decreasedPCRduplicationratesandverylowbias.*ThisresultsinlowerduplicationratesandimprovedcoverageofGC-andATrichregions,promoters,lowcomplexityandotherchallengingregionsinallNGSlibraryconstructionworkflowsrequiringlibraryamplification.

Inadditiontothestandardlibraryamplificationformulation,KAPAHiFiisavailableinauniquereal-timeformulation,forapplicationsthatdemandprecisecontroloverlibraryamplification.Auracil-tolerantvariant,KAPAHiFiUracil+isalsoavailableforthehigh-efficiency,high-fidelity,low-biasamplificationoflibrariesconstructedfrombisulfite-treatedDNA.

KAPALibraryAmplificationKitsforIllumina® platformsnowalsoincludeanoptimallyformulatedLibraryAmplificationPrimerMix.

*Dataonfile.

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Achievethelowestamplificationbiasandduplicationrates

  • Loweramplificationbiasresultinimprovedrepresentationofalllibraryfragmentsandsequenceregions
  • High-efficiencyamplificationleadstofeweramplificationcyclesandlowerPCRduplicates
  • Lessadditionalnext-generationorSangersequencingneededtocompletegenomes
The first exons of many genes have a high GC content, and are therefore difficult to amplify. Regions of these exons are therefore typically underrepresented in whole exome sequence data. Pre-capture amplification with the evolved, low-bias KAPA HiFi DNA Polymerase results in significantly improved coverage of GC- and AT-rich regions, and other sequence elements that are difficult to amplify. Data courtesy of The Broad Institute. Data on file.

ImprovecoverageofGC-andAT-richregions

  • LoweramplificationbiasimprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions
  • ImprovedoverallcoverageandcoverageuniformityobservedonbothIllumina andIonTorrent™ sequencingplatforms
Real-time, high fidelity amplification of multiple libraries with the KAPA Real-Time Library Amplification Kit. Twenty libraries, spanning a ~64-fold concentration range (equivalent to 6 cycles), were simultaneously amplified and terminated after 14 cycles. Fourteen of the twenty libraries fall within the targeted amplification range (the fluorescence range defined by the Real-Time Amplification Standards 1¬–3). The remaining six libraries could either be used as is, noting that they may be outside the optimal concentration range, or they could be re-amplified individually or in high- or low-concentration groups. Data on file.

Exerciseprecisecontroloverlibraryamplification

  • KAPAReal-TimeAmplificationKitsallowforlibraryamplificationtobeobservedinrealtime
  • Terminateamplificationattheoptimalpointforindividualsamples
  • Optimizelibraryamplificationparametersforhigherthroughputworkflows
Error rates of Taq DNA polymerase and polymerases typically used in NGS library amplification. The error rates of proofreading DNA polymerases such as Q5 (New England Biolabs), Phusion (Thermo Scientific) and the evolved KAPA HiFi DNA Polymerase is 50 – 100 times lower than that of Taq. The error rate of KAPA HiFi was confirmed by deep sequencing on the 454 platform, and is 1 error per 3.54 x 106 nucleotides incorporated. Data on file.

AmplifyNGSlibrarieswithindustry-leADIngfidelity

  • KAPAHiFiwasengineeredusingdirectedevolutiontohaveenhancedproofreading(3′-5′exonuclease)activity
  • Industry-leadingfidelityconfirmedby454sequencing

Applications:

Applications
  • AmplificationofIllumina libraries(DNAandRNAsequencing)
  • Real-timeamplificationoflibrariespreparedfromlowinput,ChIPDNAandotherprecioussamples
  • Pre-andpost-captureamplificationintargetedcaptureworkflows

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

KitsincludeKAPAHiFiHotStartReadyMix(2X),aconvenientPCRmastermixcontainingKAPAHiFiHotStartDNAPolymerase,KAPAdNTPs,reactionbuffer,andMgCl2atafinalconcentrationof2.5mM.Kitswithprimersarealsoavailable.

Kitsforreal-timelibraryamplificationincludeareal-timeversionoftheKAPAHiFiHotStartReadyMix,aswellasfluorescentstandardstomonitorlibraryamplification.

Components

LAK_Component Chart

Specifications

Spec
Description
CompatIBLePlatform
IlluminaHiSeq,MiSeq,NextSeq,andGAIIx
LibraryType
DNA,RNA,andbisulfite-treatedDNA
StartingMaterial
AnyNGSlibrarythatrequiresamplification
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)RNA-SeqChIP-SeqAmpliconSequencing
品牌介绍

Kapabiosystems


Kapa是世界上著名的生物技术公司,总部位于美国。特色产品:Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。


We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.


产品列表:



No.

品牌

货号

名称

规格

1

kapa

KK4824

Complete kit (Universal)

500 x 20 µL reactions

2

kapa

KK4854

Complete kit (optimized for Roche® LightCycler 480)

500 x 20 µL reactions

3

kapa

KK4873

Complete Kit (ROX Low)

500 x 20 µL reactions

4

kapa

KK4973

qPCR MasterMix (ROX Low ) and Primer Premix only

500 x 20 µL reactions

5

kapa

KK4827

Complete kit (Universal)

500 x 20 µL reactions

6

kapa

KK4847

Complete kit (Bio-Rad®)

500 x 20 µL reactions

7

kapa

KK4924

qPCR MasterMix (Universal) and Primer Premix only

500 x 20 µL reactions

8

kapa

KK4944

qPCR MasterMix (Bio-Rad) and Primer Premix only

500 x 20 µL reactions

9

kapa

KK4813

Library Quantification Primer Premix

500 µL

10

kapa

KK2502

HotStart PCR Kit, with dNTPs

250U

11

kapa

KK2102

With dNTPs

250U

12

kapa

KK2602

HotStart ReadyMix, 500 x 25 µL reactions

6.25 mL

13

kapa

KK4701

100 x 20 µL reactions (Universal)

1 ml

14

kapa

KK4705

100 x 20 µL reactions, ROX (ABI Prism®)

1 ml

15

kapa

KK4709

100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)

1 ml


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