Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8000/5 mL,Kapa biosystems,,Kapa biosystems,Kapa,kapabiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8000/5 mL

产品编号：KK8000

市场价：¥3000.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>RNA文库制备试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$150.00

品牌： Kapa biosystems

公司：Kapa Biosystems.

公司分类：

商品介绍

Description:

KAPAPureBeads(5mL)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

Upto99.98%rRNAdepletion*
FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
Robustandreproducibleacrossvariousinputamounts
Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleADIngrRNAdepletion

SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest

High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.

Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.

Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.

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Unsurpassedsequencingdataquality

Detectionofmoregenesanduniquetranscripts
Accurateandclearidentificationofsplicesitesandalternativegenesplicing
ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts

Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.

0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile."> Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.

Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.

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Highlyreproduciblesequencingresults

Highcorrelationevenbetweendifferenttestingconditions
Lowsample-to-samplevariationformorereliableresults

Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.

Minimal positional bias. With 100 ng of high-quality RNA input, 3

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Improvedcoverageuniformity

Uniformdistributionofreadsovereachtranscript
Minimal5’–3’biasacrosstranscripts

_*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAStrandedmRNA-SeqKits

KAPARNAHyperPrepKitswithRiboErase

KAPAmRNAHyperPrepKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

WholeTranscriptome
GeneExpression
TranscriptDiscoveryandAnnotation
TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

Specifications

CompatiblePlatform

IlluminaHiSeq®,NextSeq®andMiSeq®

LibraryType

RNA

StartingMaterial

High-qualityTotalRNA

InputAmount

100ng-1µg

品牌介绍

Kapabiosystems

Kapa是世界上著名的生物技术公司，总部位于美国。特色产品：Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。

We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.

产品列表：

No.	品牌	货号	名称	规格
1	kapa	KK4824	Complete kit (Universal)	500 x 20 µL reactions
2	kapa	KK4854	Complete kit (optimized for Roche® LightCycler 480)	500 x 20 µL reactions
3	kapa	KK4873	Complete Kit (ROX Low)	500 x 20 µL reactions
4	kapa	KK4973	qPCR MasterMix (ROX Low ) and Primer Premix only	500 x 20 µL reactions
5	kapa	KK4827	Complete kit (Universal)	500 x 20 µL reactions
6	kapa	KK4847	Complete kit (Bio-Rad®)	500 x 20 µL reactions
7	kapa	KK4924	qPCR MasterMix (Universal) and Primer Premix only	500 x 20 µL reactions
8	kapa	KK4944	qPCR MasterMix (Bio-Rad) and Primer Premix only	500 x 20 µL reactions
9	kapa	KK4813	Library Quantification Primer Premix	500 µL
10	kapa	KK2502	HotStart PCR Kit, with dNTPs	250U
11	kapa	KK2102	With dNTPs	250U
12	kapa	KK2602	HotStart ReadyMix, 500 x 25 µL reactions	6.25 mL
13	kapa	KK4701	100 x 20 µL reactions (Universal)	1 ml
14	kapa	KK4705	100 x 20 µL reactions, ROX (ABI Prism®)	1 ml
15	kapa	KK4709	100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)	1 ml