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DNA扩增、二代测序、分子诊断
℡ 4000-520-616
℡ 4000-520-616
Kapa biosystems/KAPA Library Quantification Kits/KK4944/500 x 20 µL reactions
产品编号:KK4944
市  场 价:¥9460.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$473.00
品      牌: Kapa biosystems
公      司:Kapa Biosystems.
公司分类:
Kapa biosystems/KAPA Library Quantification Kits/KK4944/500 x 20 µL reactions
商品介绍

Description:

qPCRMasterMix(Bio-Rad)andPrimerPremixonly

KAPALibraryQuantificationKits

forNext-GenerationSequencing

Ourstandardsdon’tchange.Neithershouldyours.

KAPALibraryQuantificationKitscontainallthereagentsneededfortheaccurate,reliableandreproducIBLeqPCR-basedquantificationofNGSlibrariespriortopoolingforcaptureorflowcellamplification.KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR.TheengineeredKAPASYBRFASTDNAPolymerasecontainedintheMasterMixamplifiesGC-andAT-richDNAfragmentsofdifferentlengthswithsimilarefficiency,makingittheonlyqPCRMasterMixcapableofaccurateqPCR-basedquantificationofNGSlibraries.*Thepre-dilutedsetofDNAStandardscontainedineachkitiscarefullyquality-controlledtoensurelot-to-lotconsistencyandavoiddatadriftovertime.OptimizedPrimerPremixesensureoptimalPCRefficiency.

KitswithDNAStandardsandPrimerPremixesforIllumina® andIonTorrent™sequencers areavailable.Pleasereferencethe InstrumentCompatibilityChart forguidanceoncompatibleplatforms.

NEW!ImproveexperimentaldesignswithourTechnicalGuideforIlluminaPlatforms


*Dataonfile.

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

AccuratelyquantifyPCR-competentsequencingtemplates

  • qPCRonly“counts”thoselibrarymoleculesthatcanbesequenced
  • Moreconsistentclusterdensitiesortemplate-to-beadratiosenableoptimalutilizationofsequencingresources
Equimolar pooling of indexed libraries. Eleven indexed Illumina TruSeq libraries were quantified by qPCR using the KAPA Library Quantification Kit, and then combined to achieve equal final concentrations in two separate pools for multiplexed sequencing on different flow-cell lanes. The eleven libraries ranged ~11-fold in concentration from 0.67 pM to 7.65 pM, while representation of each index varied between 90% and 127% of expected assigned reads per lane. Data on file.

Improvepoolingformultiplexedsequencing

  • LibraryconcentrationsdeterminedbyqPCRaremorereliablecomparedtoothermethods*
  • TheengineeredKAPASYBRFASTqPCRMasterMixenablesaccuratepoolingoflibrarieswithextremeGC-contentsorlongerinserts*
Nine human DNA libraries and two microbial DNA libraries (Rhodococcus sp. ~70% GC and Staphylococcus sp. ~35% GC) were used to compare quantification results obtained with distinct lots (”Lot 1” and “Lot 2”), and distinct sets of reagents from the same lot (”Set 1” and “Set 2”) of KAPA Library Quantification Kits for Illumina platforms. Data on file.

Eliminatedriftwithcarefullyquality-controlledDNAStandards

  • PredilutedDNAStandardseliminatequantificationerrorsassociatedwithpreparationofstandardcurvesusingin-housestandards*
  • KAPADNAStandardsundergostrictqualitycontroltoensurelot-to-lotconsistencyandeliminatedatadriftovertime
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Improvethroughputwithautomation

  • Libraryquantificationassayiscompatiblewith96-and384-wellformat
  • Librarydilution,reactionsetupanddataanalysiscanbeautomatedforHTPpipelines
  • LearnMore aboutKAPAAutomatedSolutions

*Dataonfile.

RelatedProducts

Normalizinglibrariesforpoolingandclusteramplification?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • Quantificationoflibrariespriortopoolingformultiplexedsequencing
  • QuantificationofindividuallibrariesorlibrarypoolspriortoclusteramplificationoremPCR
  • Quantificationoflibrariespriortoandafterhybridizationcapture
  • Qualitycontrol,optimizationandtroubleshootingoflibraryconstructionprocessesorworkflows

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

CompletekitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(10X)andasetof6DNAStandards.ReagentsforqPCR(KAPASYBRFASTqPCRMasterMixandPrimerPremix)andDNAStandards(1–6)arealsosoldseparately.Wherenoted,MasterMixescontaininstrument-specificreferencedyes,whiletheUniversalkitincludesROXHighandROXLow(both50X)separately.

Components–Illumina®Platform

Components–IonTorrent™Platform

LQK-Ion_Component Chart

Specifications

Spec
Description
CompatiblePlatforms
IlluminaHiSeq,NextSeq,MiSeq,andGAIIxIonTorrentProtonandPGM454GSFLX+andGSJuniorSOLiD5500series
LibraryType
Any
StartingMaterial
AnyNGSlibraryreadyforclusteramplificationoremPCR
Standardcurveconcentrationrange
20–0.0002pMforIlluminalibraries83–0.00083pMforIonTorrentand454libraries10–0.0001pg/µLforSOLiDlibraries
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)RNA-SeqChIP-SeqAmpliconSequencingMethyl-Seq
品牌介绍

Kapabiosystems


Kapa是世界上著名的生物技术公司,总部位于美国。特色产品:Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。


We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.


产品列表:



No.

品牌

货号

名称

规格

1

kapa

KK4824

Complete kit (Universal)

500 x 20 µL reactions

2

kapa

KK4854

Complete kit (optimized for Roche® LightCycler 480)

500 x 20 µL reactions

3

kapa

KK4873

Complete Kit (ROX Low)

500 x 20 µL reactions

4

kapa

KK4973

qPCR MasterMix (ROX Low ) and Primer Premix only

500 x 20 µL reactions

5

kapa

KK4827

Complete kit (Universal)

500 x 20 µL reactions

6

kapa

KK4847

Complete kit (Bio-Rad®)

500 x 20 µL reactions

7

kapa

KK4924

qPCR MasterMix (Universal) and Primer Premix only

500 x 20 µL reactions

8

kapa

KK4944

qPCR MasterMix (Bio-Rad) and Primer Premix only

500 x 20 µL reactions

9

kapa

KK4813

Library Quantification Primer Premix

500 µL

10

kapa

KK2502

HotStart PCR Kit, with dNTPs

250U

11

kapa

KK2102

With dNTPs

250U

12

kapa

KK2602

HotStart ReadyMix, 500 x 25 µL reactions

6.25 mL

13

kapa

KK4701

100 x 20 µL reactions (Universal)

1 ml

14

kapa

KK4705

100 x 20 µL reactions, ROX (ABI Prism®)

1 ml

15

kapa

KK4709

100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)

1 ml


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