Kapa biosystems/KAPA mRNA HyperPrep Kits/KK8712/12 adapters x 40 µl each,Kapa biosystems,,Kapa biosystems,Kapa,kapabiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Kapa biosystems/KAPA mRNA HyperPrep Kits/KK8712/12 adapters x 40 µl each

产品编号：KK8712

市场价：¥9600.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>RNA文库制备试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$480.00

品牌： Kapa biosystems

公司：Kapa Biosystems.

公司分类：

商品介绍

Description:

KAPASingle-IndexedAdapterKit,SetB(1.5µM)

KAPAmRNAHyperPrepKits

Single-DayRNA

TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.

Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

single-daylibraryconstruction,inclusiveofmRNAcapture
reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
flexibleinputof50ng–1µgtotalRNA*
maintainover99%strandspecificity*
KAPAPureBeadsincludedforreactionpurifications

KAPARNAHyperPrepKits

KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.

DownloadourAdapterandBeadCalculator

^*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA mRNA HyperPrep Kit results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

With an equivalent amount of sequencing, more genes and unique transcripts are identified using the KAPA mRNA HyperPrep Kit in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

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Sequencewhatmatters

WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
Identifymoreuniquetranscriptsandgeneswithequivalentsequencing

For the top 1000 transcripts, the KAPA mRNA Hyper Prep Kit results in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes, along with improved splice junction and antisense transcript detection was demonstrated using the KAPA mRNA Hyper Prep Kit in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

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Achievesuperiorcoverageuniformity

Obtainmoreuniformdistributionofreadsacrosstranscripts
ImprovecoverageofdifficultGC-richtranscripts

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPARNAHyperPrepKitswithRiboErase

KAPAStrandedmRNA-SeqKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpressionanalysis
Detectionofgenefusions,isoforms,andotherstructuralvariants
Noveltranscriptidentification,includingnoncodingtranscripts
SNVdiscovery
Wholetranscriptome(KAPARNAHyperPrepKitsonly)
Targetedtranscriptome(KAPARNAHyperPrepKitsonly)

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.

RNAHyper_Component Chart

Specifications

CompatibilityPlatform

IlluminaHiSeq,NextSeq,andMiSeq

LibraryType

RNA

StartingMaterial

RNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA

StartingMaterial

RNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg

品牌介绍

Kapabiosystems

Kapa是世界上著名的生物技术公司，总部位于美国。特色产品：Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。

We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.

产品列表：

No.	品牌	货号	名称	规格
1	kapa	KK4824	Complete kit (Universal)	500 x 20 µL reactions
2	kapa	KK4854	Complete kit (optimized for Roche® LightCycler 480)	500 x 20 µL reactions
3	kapa	KK4873	Complete Kit (ROX Low)	500 x 20 µL reactions
4	kapa	KK4973	qPCR MasterMix (ROX Low ) and Primer Premix only	500 x 20 µL reactions
5	kapa	KK4827	Complete kit (Universal)	500 x 20 µL reactions
6	kapa	KK4847	Complete kit (Bio-Rad®)	500 x 20 µL reactions
7	kapa	KK4924	qPCR MasterMix (Universal) and Primer Premix only	500 x 20 µL reactions
8	kapa	KK4944	qPCR MasterMix (Bio-Rad) and Primer Premix only	500 x 20 µL reactions
9	kapa	KK4813	Library Quantification Primer Premix	500 µL
10	kapa	KK2502	HotStart PCR Kit, with dNTPs	250U
11	kapa	KK2102	With dNTPs	250U
12	kapa	KK2602	HotStart ReadyMix, 500 x 25 µL reactions	6.25 mL
13	kapa	KK4701	100 x 20 µL reactions (Universal)	1 ml
14	kapa	KK4705	100 x 20 µL reactions, ROX (ABI Prism®)	1 ml
15	kapa	KK4709	100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)	1 ml