DNA quality impacts the fragmentation of FFPE DNA. The guidelines presented in the Technical Data Sheet are a good starting point for FFPE samples with a Q129/Q41 ratio of 0.4 or higher (as determined with the KAPA hgDNA Quantification and QC Kit). However, longer fragmentation times may improve results for lower-quality FFPE samples. Longer fragmentation times typically increase the proportion of input DNA converted to fragments in the 150 – 250 bp range, reduce residual high-molecular weight DNA, and correlate with higher yields during library construction.
2017-12-21