Kapa biosystems/KAPA Stranded mRNA-Seq Kits/KK8002/60 mL,Kapa biosystems,,Kapa biosystems,Kapa,kapabiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Kapa biosystems/KAPA Stranded mRNA-Seq Kits/KK8002/60 mL

产品编号：KK8002

市场价：¥14400.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>RNA文库制备试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$720.00

品牌： Kapa biosystems

公司：Kapa Biosystems.

公司分类：

商品介绍

Description:

KAPAPureBeads(60mL)

KAPAStrandedmRNA-SeqKits

Evendifficultmessagesshouldbeunderstood.

KAPAStrandedmRNA-SeqKitsincludealltheenzymesandbuffersrequiredforCDNAlibrarypreparationforIlluminaNext-GenerationSequencing,utilizing100ng–4µgoftotalRNA.KAPAmRNACaptureBeadsareincludedforisolationofpoly(A)-tailedRNA.Kitsprovideprecisemeasurementofstrandorientation(>99%),uniformcoverage,andhigh-confidencemappingofalternatetranscripts,andareoptimizedfortheimprovedcoverageofGC-richandlow-abundancetranscripts.*KitscontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,aswellasKAPAmRNACaptureBeadsandastreamlined,“with-bead”protocol.

KAPAStrandedRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,butdonotcontaintheKAPAmRNACaptureBeads.Kitscanbeusedtopreparelibrariesfrom10–400ngofeitherpoly(A)-selected,ribosomallydepleted,ortotalRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Download AdapterandBeadCalculator

_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Improved coverage of GC-rich transcripts. The 5

Uncoverchallengingtranscripts

ImprovedcoverageofGC-richtranscripts
Enhancedidentificationofexonicregions

Improved coverage of low-abundance transcripts. GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the KAPA Stranded mRNA-Seq Kit (green) at 500 ng input total RNA (outlined in red). In comparison, Illumina TruSeq Stranded mRNA Sample Prep Kit (orange) shows coverage gaps, even with higher inputs (4 μg). Data on file.

Detectlow-abundancetranscripts

Enablesidentificationoftranscriptsmissedbycompetitorkits,evenwithhighinput*
Highuniformityacrossvaryingamountsofsampleinput*

30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile."> Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Identifymoregenes

HigherpercentageofuniquelymappedreadscomparedtoIllumina TruSeq™StrandedmRNASamplePrepKits*
Lowerduplicationratesyieldbettercoverage

Minimal positional bias. With intact, high-quality RNA input, 3

Maintainhighcoverageuniformity

Minimal5’–3′biasacrosstranscripts
Moreuniformdistributionofreadsovereachtranscript

_*Dataonfile.

RelatedProducts

SequencingchallengingRNAsamples?HowaboutDNA?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpression
Singlenucleotidevariation(SNV)discovery
Post-transcriptionalSNVs
Fusiongeneidentification
Targetedtranscriptome
Wholetranscriptome

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto8 months at-20˚C. KAPAmRNACaptureBeadscanbestoredforupto8months at-4˚C.

Components

mRNA_Component Chart

Specifications

CompatIBLePlatform

IlluminaHiSeq,NextSeq,MiSeqandGAIIx

StartingMaterial

TotalRNA,mRNA,orribosomaldepletedRNA

InputAmount

StrandedmRNA-SeqKits:100ng–4µgStrandedRNA-SeqKits:10ng–400ng

品牌介绍

Kapabiosystems

Kapa是世界上著名的生物技术公司，总部位于美国。特色产品：Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。

We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.

产品列表：

No.	品牌	货号	名称	规格
1	kapa	KK4824	Complete kit (Universal)	500 x 20 µL reactions
2	kapa	KK4854	Complete kit (optimized for Roche® LightCycler 480)	500 x 20 µL reactions
3	kapa	KK4873	Complete Kit (ROX Low)	500 x 20 µL reactions
4	kapa	KK4973	qPCR MasterMix (ROX Low ) and Primer Premix only	500 x 20 µL reactions
5	kapa	KK4827	Complete kit (Universal)	500 x 20 µL reactions
6	kapa	KK4847	Complete kit (Bio-Rad®)	500 x 20 µL reactions
7	kapa	KK4924	qPCR MasterMix (Universal) and Primer Premix only	500 x 20 µL reactions
8	kapa	KK4944	qPCR MasterMix (Bio-Rad) and Primer Premix only	500 x 20 µL reactions
9	kapa	KK4813	Library Quantification Primer Premix	500 µL
10	kapa	KK2502	HotStart PCR Kit, with dNTPs	250U
11	kapa	KK2102	With dNTPs	250U
12	kapa	KK2602	HotStart ReadyMix, 500 x 25 µL reactions	6.25 mL
13	kapa	KK4701	100 x 20 µL reactions (Universal)	1 ml
14	kapa	KK4705	100 x 20 µL reactions, ROX (ABI Prism®)	1 ml
15	kapa	KK4709	100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)	1 ml