Kapa biosystems/KAPA Hyper Prep Kits/KK8701/12 adapters x 40 µl each,Kapa biosystems,,Kapa biosystems,Kapa,kapabiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Kapa biosystems/KAPA Hyper Prep Kits/KK8701/12 adapters x 40 µl each

产品编号：KK8701

市场价：¥18720.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>DNA文库制备试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$936.00

品牌： Kapa biosystems

公司：Kapa Biosystems.

公司分类：

商品介绍

Description:

KAPASingle-IndexedAdapterKit,SetA(30µM)

KAPAHyperPrepKits

Shiftyourworkflowintohyperdrive.

TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina^®sequencing.

Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 μg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 μg of amplified library required for target capture. Data on file.

Number of amplification cycles needed to generate ≥1 µg amplified library for target capture with the SeqCap EZ Comprehensive Cancer Design (578 genes, 4 MB; Roche Nimblegen), for libraries prepared with each kit. The higher conversion rates achieved with the KAPA Hyper Prep Kit, combined with high-efficiency, low-bias amplification with KAPA HiFi DNA Polymerase, translates to significantly less cycles of pre-capture amplification and subsequent improvements in sequence data quality. Data on file.

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Improvelibraryyieldsandsequencequality

Higherlibraryyieldstranslatetogreatermolecularcomplexity
FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage

Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage. Data on file.

Conversion rates for FFPE DNA has been identified as a primary bottleneck in NGS library construction. With the KAPA Hyper Prep Kit, conversion rates for FFPE samples are similar to those for high-quality DNA. This leads to significantly lower duplication rates and higher overall target coverage in exome and other target capture workflows. Conversion data are for libraries prepared from 80 – 100 ng. Covaris-sheared FFPE DNA of variable quality, or 100 ng control DNA. Data on file.

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Createhigh-qualitylibrariesfromFFPEsamples

Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
Significantlylowerduplicationratesandhighercoverage*

The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing. Data on file.

Completelibraryconstructioninlessthan3hours

One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility

Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity. Data on file.

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Improveperformancewithlow-inputsamples

Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
Increaseyieldswithoutinducingsizebias

_*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPALibraryQuantificationKits

KAPAStrandedmRNA-SeqKits

Applications:

Applications

WholeGenomeSequencing
WholeExomeSequencing
TargetedSequencing(custompanels)
ChIP-seq
AmpliconSequencing
Methyl-seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix

Components

Hyper_Component Chart

Specifications

CompatIBLePlatform

lluminaHiSeq,MiSeq,NextSeqandGAIIx

LibraryType

DNA

StartingMaterial

FragmentedDNA,CDNA,orPCRamplicons

InputAmount

1ng–1µg

品牌介绍

Kapabiosystems

Kapa是世界上著名的生物技术公司，总部位于美国。特色产品：Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。

We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.

产品列表：

No.	品牌	货号	名称	规格
1	kapa	KK4824	Complete kit (Universal)	500 x 20 µL reactions
2	kapa	KK4854	Complete kit (optimized for Roche® LightCycler 480)	500 x 20 µL reactions
3	kapa	KK4873	Complete Kit (ROX Low)	500 x 20 µL reactions
4	kapa	KK4973	qPCR MasterMix (ROX Low ) and Primer Premix only	500 x 20 µL reactions
5	kapa	KK4827	Complete kit (Universal)	500 x 20 µL reactions
6	kapa	KK4847	Complete kit (Bio-Rad®)	500 x 20 µL reactions
7	kapa	KK4924	qPCR MasterMix (Universal) and Primer Premix only	500 x 20 µL reactions
8	kapa	KK4944	qPCR MasterMix (Bio-Rad) and Primer Premix only	500 x 20 µL reactions
9	kapa	KK4813	Library Quantification Primer Premix	500 µL
10	kapa	KK2502	HotStart PCR Kit, with dNTPs	250U
11	kapa	KK2102	With dNTPs	250U
12	kapa	KK2602	HotStart ReadyMix, 500 x 25 µL reactions	6.25 mL
13	kapa	KK4701	100 x 20 µL reactions (Universal)	1 ml
14	kapa	KK4705	100 x 20 µL reactions, ROX (ABI Prism®)	1 ml
15	kapa	KK4709	100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™)	1 ml