KAPAhgDNAQuantificationandQCKitscontainallthereagentsneededfortheqPCR-basedquantificationandqualityassessmentofhumangenomicDNAsamplespriortoNGSlibraryconstruction.
KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR,aswellasapre-dilutedsetofDNAstandardsandprimerpremixestargetingdifferentportionsofahighlyconservedsingle-copyhumanlocus.Absolutequantificationisachievedwiththeprimerpairdefiningtheshortestfragment,whereastheadditionalprimersareusedtoderiveinformationabouttheamountofamplifiabletemplateintheDNAsample.Qualityscores(orQ-ratios)generatedwiththekitmaybeusedtopredicttheoutcomeoflibraryconstruction,ortailorworkflowsforsamplesofvariablequality,particularlyFFPEDNA.
ThemethodiseasytoautomateandcanbeappliedtoanyprocessorworkflowthatrequiresaccuratequantificationofdiluteDNAsamples,orsamplesthatmaycontainahighproportionofDNAthatisrecalcitranttoPCRamplification.
Downloadour KAPAhgDNAQuantificationandQCDataAnalysisTemplate.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Kitscanbestoredforupto12months at-20˚C.
Complete(MasterMix)kitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(41bp,129bpand305bp,10X)andasetof5DNAStandards.PrimerPremixesandDNAStandardsarealsosoldseparately.
Kapabiosystems
Kapa是世界上著名的生物技术公司,总部位于美国。特色产品:Kapa2G快速PCR、实时荧光定量PCR、Kapa长片段DNA多聚酶、Kapa高保真DNA多聚酶。
We are constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Our core protein engineering technologies enable us to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.
产品列表:
No. | 品牌 | 货号 | 名称 | 规格 |
1 | kapa | KK4824 | Complete kit (Universal) | 500 x 20 µL reactions |
2 | kapa | KK4854 | Complete kit (optimized for Roche® LightCycler 480) | 500 x 20 µL reactions |
3 | kapa | KK4873 | Complete Kit (ROX Low) | 500 x 20 µL reactions |
4 | kapa | KK4973 | qPCR MasterMix (ROX Low ) and Primer Premix only | 500 x 20 µL reactions |
5 | kapa | KK4827 | Complete kit (Universal) | 500 x 20 µL reactions |
6 | kapa | KK4847 | Complete kit (Bio-Rad®) | 500 x 20 µL reactions |
7 | kapa | KK4924 | qPCR MasterMix (Universal) and Primer Premix only | 500 x 20 µL reactions |
8 | kapa | KK4944 | qPCR MasterMix (Bio-Rad) and Primer Premix only | 500 x 20 µL reactions |
9 | kapa | KK4813 | Library Quantification Primer Premix | 500 µL |
10 | kapa | KK2502 | HotStart PCR Kit, with dNTPs | 250U |
11 | kapa | KK2102 | With dNTPs | 250U |
12 | kapa | KK2602 | HotStart ReadyMix, 500 x 25 µL reactions | 6.25 mL |
13 | kapa | KK4701 | 100 x 20 µL reactions (Universal) | 1 ml |
14 | kapa | KK4705 | 100 x 20 µL reactions, ROX (ABI Prism®) | 1 ml |
15 | kapa | KK4709 | 100 x 20 µL reactions, with fluorescein (Bio-Rad® iCycler™) | 1 ml |